Evaluation of DNA lesions induced by high-let ion beams using quantitative polymerase chain reaction

Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Sakamoto, Ayako; Shimizu, Kikuo*

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 104, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

no abstracts in English

Molecular analysis of heavy ion induced mutations in budding yeast

Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Sakamoto, Ayako; Nozawa, Shigeki; Narumi, Issey*; Shimizu, Kikuo*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 112, 2014/03

Fundamental study on molecular mechanism underlying repair of heavy-ion induced DNA damage in the

Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Sakamoto, Ayako; Nozawa, Shigeki; Narumi, Issei; Shimizu, Kikuo*

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 108, 2012/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2011-043.pdf

Molecular analysis of carbon ion induced mutations in the yeast and mutants

Matsuo, Yoichiro*; Nishijima, Shigehiro*; Hase, Yoshihiro; Nozawa, Shigeki; Sakamoto, Ayako; Narumi, Issei; Shimizu, Kikuo*

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 75, 2009/12

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2009-041.pdf

no abstracts in English

Study on molecular mechanism of high-LET carbon ion beam induced mutations in

Matsuo, Yoichiro*; Nishijima, Shigehiro*; Hase, Yoshihiro; Sakamoto, Ayako; Narumi, Issei; Shimizu, Kikuo*

JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 60, 2008/11

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2008-055.pdf

no abstracts in English

AtREV1, a Y-family DNA polymerase in Arabidopsis, has deoxynucleotidyl transferase activity

Takahashi, Shinya*; Sakamoto, Ayako; Tanaka, Atsushi; Shimizu, Kikuo*

Plant Physiology, 145(3), p.1052 - 1060, 2007/11

https://doi.org/10.1104/pp.107.101980

To clarify the functions of AtREV1 protein, we expressed it in E. coli and purified it. The deoxynucleotidyl transferase activity of the recombinant AtREV1 was examined in a primer extension assay . The recombinant AtREV1 transferred one or two nucleotides to the primer end. Especially, it efficiently inserted dCMP regardless of the opposite base. AtREV1 also inserted a dCMP opposite the apurinic/apyrimidinic (AP) sites, which are physiologically generated or induced by various DNA-damaging agents. However, AtREV1 had no insertion activities against UV-inducible DNA lesions. Although the substrate specificity of AtREV1 was rather narrow in the presence of magnesium ion, it widened in the presence of manganese ion. These results suggest that AtREV1 serves as a deoxycytidyl transferase in plant cells.

Specificity of mutations induced by carbon ions in budding yeast

Matsuo, Yuichiro*; Nishijima, Shigehiro*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Shimizu, Kikuo*

Mutation Research; Fundamental and Molecular Mechanisms of Mutagenesis, 602(1-2), p.7 - 13, 2006/12

https://doi.org/10.1016/j.mrfmmm.2006.07.001

no abstracts in English

Mutation of budding yeast induced by ion beam irradiation

Izumi, Yoshinobu*; Matsuo, Yoichiro*; Sakamoto, Ayako; Takagi, Keiichi*; Hatashita, Masanori*; Kojima, Takao*; Shimizu, Kikuo*

no journal, , 

In cases of ion-beam irradiations, several features of deep interest can be observed, contrasting with the low-LET radiations such as -rays. In order to elucidate the induction mechanisms of mutations peculiar to ion beams, carbon-ion beams with LETs of 13 keV/m - 107 keV/m and proton beam with LET of 0.45 keV/m were irradiated to budding yeast. Carbon-ion beams and proton beam irradiation were performed in TIARA or HIMAC, and WERC, respectively. After irradiations, survival ratios and mutation frequencies were analyzed as well as sequencing and gene expression analyses. In order to elucidate the molecular mechanisms of mutagenesis caused by radiations, we used (BER-) which is deficient in the elimination of 8-oxodGTP and (MMR-) deficient in the mismatch-repair function. For comparison, we also used (NHEJ-) and (HR-) that were deficient in the double-strand breaks (DSBs)-repair function.

Mechanisms of mutation induction in budding yeast by heavy ion irradiation

Matsuo, Yoichiro*; Nishijima, Shigehiro*; Shimizu, Kikuo*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi

no journal, , 

no abstracts in English

Analysis of mutagenesis mechanisms of budding yeast by carbon ion beam irradiation

Matsuo, Yoichiro*; Nishijima, Shigehiro*; Hase, Yoshihiro; Sakamoto, Ayako; Narumi, Issei; Shimizu, Kikuo*

no journal, , 

no abstracts in English