Non-invasive imaging of carbon translocation and nitrogen fixation in intact plants using the positron-emitting tracer imaging system

ポジトロンイメージング技術を用いた炭素転流及び窒素固定の非侵襲的イメージング

鈴井 伸郎; 石井 里美; 河地 有木; 伊藤 小百合; 中村 進一*; 石岡 典子; 藤巻 秀

Suzui, Nobuo; Ishii, Satomi; Kawachi, Naoki; Ito, Sayuri; Nakamura, Shinichi*; Ishioka, Noriko; Fujimaki, Shu

We developed analytical methods for monitoring carbon translocation and nitrogen fixation in intact plants using short-lived radioactive tracer gases and the positron-emitting tracer imaging system (PETIS). In the analysis of carbon translocation, we fed C (half life: 20.4 min)-labeled radioactive carbon dioxide gas to leave blades of rice plants, and serial images of C-photoassimilate were obtained non-invasively using PETIS. In order to understand source-sink relations, we manipulated source and sink strength by treating tested rice plants with p-chlorobenzenesulfonic acid (PCMBS), an inhibitor of sucrose transporters. As a result, a decrease in the velocity after the manipulation was successfully detected. In the analysis of nitrogen fixation, we newly developed a rapid method to produce and purify N (half life: 10.0 min)-labeled radioactive nitrogen gas and fed the gas to the underground part of nodulated soybean plants. As a result, obvious signal of N was observed at the nodules.