Comparison of Cd absorption and accumulation between transgenic tobacco plants and control plants

亜鉛トランスポーターを高発現させた形質転換タバコにおけるカドミウム動態の解析

中村 進一*; 鈴井 伸郎; 石井 里美; 河地 有木; 石岡 典子; 頼 泰樹*; 服部 浩之*; 茅野 充男*; 藤巻 秀

Nakamura, Shinichi*; Suzui, Nobuo; Ishii, Satomi; Kawachi, Naoki; Ishioka, Noriko; Rai, Hiroki*; Hattori, Hiroyuki*; Chino, Mitsuo*; Fujimaki, Shu

Phytoremediation is one of good technique to recover Cd from contaminated soils. To make this technique more effective, it is necessary to understand the mechanism of Cd dynamics and control them. It is known that Cd movement in the plant body is similar to zinc (Zn) movement. However, these mechanisms are not fully understood so far. In this work, we compared Cd absorption and accumulation between control tobacco plants and transgenic tobacco plants, which were transform with zinc transporter genes, by using positron emitting tracer imaging system (PETIS) and Cd. We succeeded to obtain fine serial images of Cd absorption and accumulation in tobacco plants, but there was no difference in Cd absorption and accumulation between transgenic tobacco plants and control plants. In previous experiments using non-radioactive Cd, Cd content in transgenic plants was 1.2 times higher than that in control plants. However, the PEITIS results indicated that such differences were not identified clearly in serial images obtained by PETIS experiments. Because PETIS experiments were performed in low Cd condition (0.1 M), we supposed that Cd concentration might have a significant impact on these results.