Developing protocol for screening of salt/drought tolerant mutants by the combination of regeneration and ionizing-radiation mutagenesis to poplar ( L. var. Koehne)

試験管内再分化と電離放射線による変異誘発を組合せた塩/乾燥耐性ポプラ( L. var. Koehne)探索のためのプロトコールの開発

Biswas, K.; 毛利 武*; 古川原 聡*; 長谷 純宏; 鳴海 一成; 大野 豊

Biswas, K.; Mori, Takeshi*; Kogawara, Satoshi*; Hase, Yoshihiro; Narumi, Issei; Ono, Yutaka

Development of suitable mutagenesis and screening system is necessary to isolate salt/drought tolerant mutants in hard wood plants, such as poplar. Here, we are introducing a specialized screening system with combination of regeneration and ion beam or ray irradiation. A high percentage of direct plant regeneration could be achieved from stem explants placed on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid and benzylaminopurine. To optimize dosage for mutagenesis, dose response relationship curve for shoot regeneration ratio had been established. Initial screening of salt/drought tolerance could be achieved by observing the ability of root development of regenerated shoots in 75 mM NaCl-supplemented rooting medium because wild-type regenerated shoots failed to develop roots in this medium. Hopefully, our system will help to reduce existing limitations of screening mutants in hard wood plants having a long life cycle.