Lombardy poplar ( L. var. ) an important woody plant species for ionizing-radiation mutagenesis

イオンビーム変異誘発のための重要な木本植物としてのポプラ

Biswas, K.; 長谷 純宏; 鳴海 一成; 大野 豊; 毛利 武*; 古川原 聡*

Biswas, K.; Hase, Yoshihiro; Narumi, Issei; Ono, Yutaka; Mori, Takeshi*; Kogawara, Satoshi*

spp. are widely employed worldwide for windbreaks and landscaping beside potential to enhance wood supplies for the plywood, hardboard, pulp, paper industries and various other purposes. Here, we present a system of screening salt/drought tolerant mutants with a combination of in vitro regeneration and ionizing-radiation mutagenesis in poplar. About 87% of the shoot explants produced shoots in shoot regeneration medium, whereas 77% regenerated shoots developed roots in root induction medium. Dose response relationship curve with in vitro regenerate shoots were establish for ion beams and rays. As shoulder point, 2.5 Gy of ion beams and 5 Gy of rays seem to be effective for mutagenic treatment to regenerate shoots.