Refine your search:     
Report No.

Functional analysis of universally conserved genes, ${it ygjD}$ and ${it yeaZ}$ orthologs, in DNA repair of ${it Deinococcus radiodurans}$

Onodera, Takefumi; Sato, Katsuya; Ota, Toshihiro*; Narumi, Issei

The ${it ygjD}$ and ${it yeaZ}$ orthologs are highly conserved in eubacteria, and the former is also widely found in the genomes of archaea and eukaryotes. ${it D. radiodurans}$, well known as a radioresistant bacterium, possesses the both orthologs, hereafter ${it DrygjD}$ and ${it DryeaZ}$. In this study, we successfully constructed ${it DrygjD}$ disruptant, ${it DryeaZ}$ disruptant and ${it DrygjD DryeaZ}$ double-disruptant strains. This result clearly indicated that neither ${it DrygjD}$ nor ${it DryeaZ}$ genes is essential for cell viability in ${it D. radiodurans}$. These gene disruptant strains exhibited extreme sensitivity to mitomycin-C compare to the wild-type strain. The deficiency of ${it DrygjD}$ and ${it DryeaZ}$ genes was complemented to restore the wild-type phenotype by introducing plasmids that express these genes.



- Accesses





[CLARIVATE ANALYTICS], [WEB OF SCIENCE], [HIGHLY CITED PAPER & CUP LOGO] and [HOT PAPER & FIRE LOGO] are trademarks of Clarivate Analytics, and/or its affiliated company or companies, and used herein by permission and/or license.