Functional analysis of universally conserved genes, and orthologs, in DNA repair of

Onodera, Takefumi; Sato, Katsuya; Ota, Toshihiro*; Narumi, Issei

The and orthologs are highly conserved in eubacteria, and the former is also widely found in the genomes of archaea and eukaryotes. , well known as a radioresistant bacterium, possesses the both orthologs, hereafter and . In this study, we successfully constructed disruptant, disruptant and double-disruptant strains. This result clearly indicated that neither nor genes is essential for cell viability in . These gene disruptant strains exhibited extreme sensitivity to mitomycin-C compare to the wild-type strain. The deficiency of and genes was complemented to restore the wild-type phenotype by introducing plasmids that express these genes.