Visualization of cell-cycle modification by ionizing irradiation in single HeLa cells using fluorescent ubiquitination-based cell-cycle indicator

FUCCI化したシングルHeLa細胞に対する放射線照射による細胞周期変異の可視化

神長 輝一; 坂本 由佳; 嘉成 由紀子; 野口 実穂; 横谷 明徳

Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Fluorescent ubiquitination-based cell cycle indicator (FUCCI) HeLa cells are one of useful model cell lines to visualize a cell-cycle because their nuclei show different colors; orange indicating G1; green indicating S/G2. In order to establish a novel assay system to study cell-cycle modification by high LET irradiation such as ion beams for cancer therapy we have observed time-lapse images of HeLa-FUCCI cells irradiated as a preliminary experiment using conventional X-rays instead of high LET ion beams. The cell-cycle was strongly arrested by irradiation at S/G2 and never progressed to G1. In contrast, cells irradiated at G1 progress to S/G2 with a similar time course as non-irradiated control cells. These results show that single FUCCI cell exposure and live cell imaging are a powerful method to trace the single cell effect of high LET irradiation on the cell-cycle in future.