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Analysis of the initial transport of fixed nitrogen and the effect of O$$_{2}$$ partial pressure on nitrogen fixation in soybean using nitrogen isotopes

窒素の同位体を用いたダイズ植物における固定窒素の輸送の解析、及び酸素分圧の窒素固定に与える影響

Hung, N. V. P.; 渡部 詩織*; 石川 伸二*; 大竹 憲邦*; 末吉 邦*; 佐藤 孝*; 石井 里美; 鈴井 伸郎; 河地 有木; 尹 永根; 藤巻 秀; 大山 卓爾*

Hung, N. V. P.; Watanabe, Shiori*; Ishikawa, Shinji*; Otake, Norikuni*; Sueyoshi, Kuni*; Sato, Takashi*; Ishii, Satomi; Suzui, Nobuo; Kawachi, Naoki; Yin, Y.-G.; Fujimaki, Shu; Oyama, Takuji*

The quantitative analysis of initial transport of fixed nitrogen in intact nodulated soybean plants was conducted by the $$^{15}$$N pulse-chase experiment. Approximately 90% of fixed $$^{15}$$N was retained in the nodules and the $$^{15}$$N distribution in the basal nodules (78%) was higher than that of in the middle (12%) and distal nodules (0.1%) after 1 hour of stable isotope $$^{15}$$N exposure. The distribution of fixed $$^{15}$$N in the nodules decreased from 90% to 7% and increased in the other part during the initial 7 hours of the chase-period. The impacts of oxygen in rhizosphere on the symbiotic nitrogen fixation in real-time was evaluated under various O$$_{2}$$ partial pressure. Soybean nodules were treated with mixed gas containing N$$_{2}$$ labeled with a radioisotope $$^{13}$$N and various concentrations of O$$_{2}$$, and the nitrogen fixation activity in the nodules was visualized by the positron-emitting tracer imaging system (PETIS). The results showed that under normal condition the nitrogen fixation ability of soybean plant was higher compared to that of under the other conditions (0% O$$_{2}$$ and 10% O$$_{2}$$). There was no significant difference in the nitrogen fixation activity between 0% O$$_{2}$$ and 10% O$$_{2}$$.

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