Enzymatic fabrication of protein-decorated gold nanoparticles by the aid of artificial peptides with gold-binding affinity

金結合性ペプチドを介したタンパク質固定化金ナノ粒子の酵素合成

二井手 哲平*; 下条 晃司郎   ; 若林 里衣*; 後藤 雅宏*; 神谷 典穂*

Niide, Teppei*; Shimojo, Kojiro; Wakabayashi, Rie*; Goto, Masahiro*; Kamiya, Noriho*

We report a new approach for the biofabrication of protein-immobilized gold nanoparticles (AuNPs), using oxidoreductase with gold-binding peptide-tagged recombinant proteins. First, we selected the A3 peptide as a gold binding moiety. The A3 peptide was introduced to the C-terminus of fusion proteins of immunoglobulin G (IgG)-binding domains of protein G and protein A. In the presence of the recombinant protein, the GLD-catalyzed cofactor reduction resulted in the efficient in situ fabrication of AuNPs immobilized with the fusion protein. Moreover, the protein-immobilized AuNPs were shown to have IgG binding activity. This enzyme-assisted one-pot methodology for protein-AuNPs conjugation offers one potent route for the facile fabrication of biomolecule-decorated metal NPs.