Effects of glutathione, applied to roots, on Cd efflux from roots in oilseed rape plants

アブラナの根に与えたグルタチオンが根からのカドミウム排出に及ぼす影響

中村 進一*; 鈴井 伸郎; 尹 永根; 石井 里美; 河地 有木; 頼 泰樹*; 服部 浩之*; 藤巻 秀

Nakamura, Shinichi*; Suzui, Nobuo; Yin, Y.-G.; Ishii, Satomi; Kawachi, Naoki; Rai, Hiroki*; Hattori, Hiroyuki*; Fujimaki, Shu

Cadmium (Cd) is one of toxic heavy metals for us. Its accumulation in crop plants causes serious health problems. Elucidation of mechanisms of Cd behaviors in plants is needed in order to reduce Cd accumulation in crop plants. In our previous work, we confirmed that glutathione (GSH), applied to roots site specifically, inhibited Cd transport from roots to shoots in oilseed rape plants. In this work, we investigated effects of GSH, applied to roots, on Cd efflux from roots in oilseed rape plants. Cd efflux from roots in these plants were also visualized and analyzed by using PETIS (Position Emitting Tracer Imaging System). Oilseed rape plants used in experiments were grown hydroponically in a green chamber under controlled growth conditions. After each treatment, Cd efflux from roots in tested plants was evaluated using an atomic absorption spectrometer. There were different effects of Cd efflux from roots on glutathione, reduced form (GSH) and oxidized form (GSSG). PETIS experiments also indicated that GSH and GSSG, applied to roots, have different effects on Cd efflux from roots.