A Model for analysis of the yield and the level of clustering of radiation-induced DNA-strand breaks in hydrated plasmids

水和プラスミドにおける放射線誘発鎖切断の収率及びクラスター化の解析モデル

鹿園 直哉; 横谷 明徳; 漆原 あゆみ; 野口 実穂; 藤井 健太郎

Shikazono, Naoya; Yokoya, Akinari; Urushibara, Ayumi; Noguchi, Miho; Fujii, Kentaro

Although it is widely accepted that the spatial distribution of strand breaks is highly relevant to the biological consequences of radiation, the extent to which strand breaks are clustered is not usually demonstrated directly from experimental data. To evaluate the spatial distribution of radiation-induced strand breaks, we have developed a model for the generation of strand breaks after irradiation. To find out whether the model is valid, we compared the calculated values to experimental data obtained by a plasmid DNA assay. Taking into account the inherent bias of the plasmid assay, the model described well the experimental results of hydrated plasmids exposed to radiation.