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${it Deinococcus radiodurans}$ YgjD and YeaZ are involved in the repair of DNA cross-links

${it Deinococcus radiodurans}$YgjDとYeaZはDNA架橋の修復に関与する

小野寺 威文; 佐藤 勝也; 太田 敏博*; 鳴海 一成

Onodera, Takefumi; Sato, Katsuya; Ota, Toshihiro*; Narumi, Issei

Orthologs of ${it Escherichia coli ygjD}$ and ${it yeaZ}$ genes are highly conserved in various organisms. The genome of the radioresistant bacterium ${it Deinococcus radiodurans}$ possesses single orthologs of ${it ygjD}$ (DR_0382) and ${it yeaZ}$ (DR_0756). Complete loss of either one or both genes did not result in any significant changes in cell growth efficiency, indicating that both genes are not essential for cell viability in ${it D. radiodurans}$, unlike the case with other species such as ${it E. coli}$, ${it Bacillus subtilis}$ and ${it Saccharomyces cerevisiae}$. Survival rates following DNA damage induced by hydrogen peroxide, $$N$$-methyl-$$N'$$-nitro-$$N$$-nitrosoguanidine, ultra violet radiation, $$gamma$$-rays, cisplatin and mitomycin C (MMC) were compared among the wild-type strain and ${it D. radiodurans ygjD}$/${it yeaZ}$ null mutants. Interestingly, the null mutants exhibited high sensitivity to MMC, which mainly causes interstrand DNA cross-links. The sensitivity of the null mutants to MMC was restored to that of the wild-type by transformation with plasmids expressing these genes. These results suggest that ${it D. radiodurans ygjD}$ and ${it yeaZ}$ genes are involved in DNA repair and play a role in the repair of DNA cross-links in ${it D. radiodurans}$.

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パーセンタイル:25.98

分野:Biochemistry & Molecular Biology

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