ヒトの浸潤性悪性脳腫瘍細胞に対する高LET重イオン誘発バイスタンダー効果

Bystander effect of high linear energy transfer charged particle radiation on human glioblastoma cells

石内 勝吾*; 長谷川 正俊*; 吉田 由香里*; 浜田 信行*; 舟山 知夫; 和田 成一*; 坂下 哲哉; 小林 泰彦; 中野 隆史*

Ishiuchi, Shogo*; Hasegawa, Masatoshi*; Yoshida, Yukari*; Hamada, Nobuyuki*; Funayama, Tomoo; Wada, Seiichi*; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Nakano, Takashi*

High linear energy transfer (LET) charged particle radiation such as C-ion beams,Ne-ion beams and Ar-ion beams is a new modality for the treatment of X-ray resistant cancers. Here we investigated the radiation-induced bystander effect on human glioblastoma cells using a CGNH-89 cell line. We irradiated individual charged-particles to target cells by a microbeam from cyclotron at JAEA Takasaki, and monitored DNA double strand breaks by H2A. X as a marker. We found bystander responses were induced in the neighboring non-irradiated cells. These results explained marked cytotoxity of heavy ions on human glioma cells.