Fundamental study on molecular mechanism underlying repair of heavy-ion induced DNA damage in the

出芽酵母における重イオン誘発DNA損傷修復の分子機構に関する基礎研究

松尾 陽一郎*; 泉 佳伸*; 長谷 純宏; 坂本 綾子; 野澤 樹; 鳴海 一成; 清水 喜久雄*

Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Sakamoto, Ayako; Nozawa, Shigeki; Narumi, Issei; Shimizu, Kikuo*

We have been studying ion-beam induced mutations in the budding yeast as a model of eukaryote cell. Yeast cells were irradiated with 220 MeV carbon ions with 107 keV/m LET. The survival rates following irradiation were determined on the basis of colony-forming ability. and strains showed hyper sensitivity, while the and strains showed relatively lower sensitivity to the carbon ion irradiation. The expression of gene was up-regulated following carbon ion irradiation but not rays. This difference may result from the repair pathway that operates in mutant strains.