検索対象:     
報告書番号:
※ 半角英数字
 年 ~ 
 年

Analysis of mutant frequencies for different LET radiations in ${it Deinococcus radiodurans}$

デイノコッカス・ラジオデュランスにおける異なるLET放射線による変異頻度の解析

佐藤 勝也; 手島 光平*; 小野寺 威文; 鳴海 一成

Sato, Katsuya; Tejima, Kohei*; Onodera, Takefumi; Narumi, Issei

${it Deinococcus radiodurans}$ exhibits extraordinary resistance to the lethal effects of ionizing radiations. This resistance has been attributed to its highly proficient DNA repair capacity. In this study, we investigated antibiotics-resistant mutant frequencies for different LET radiations in ${it D. radiodurans}$. Rifampicin is one of the antibiotics that inhibit RNA synthesis by directly interacting with the RNA polymerase beta subunit protein encoded by ${it rpoB}$ gene. By genetically altering the ${it rpoB}$ gene in genome, Rif$$^{R}$$ mutant can be generated. The Rif$$^{R}$$ mutant frequencies increased depending on radiation dose. The highest Rif$$^{R}$$ mutant frequencies were observed at a dose range that give 10$$^{-1}$$ to 10$$^{-2}$$ of surviving fraction. There is the possibility that this dose range is the best dose to generate the mutants of interest for research and breeding purposes.

Access

:

- Accesses

InCites™

:

Altmetrics

:

[CLARIVATE ANALYTICS], [WEB OF SCIENCE], [HIGHLY CITED PAPER & CUP LOGO] and [HOT PAPER & FIRE LOGO] are trademarks of Clarivate Analytics, and/or its affiliated company or companies, and used herein by permission and/or license.