Analysis of mutant frequencies for different LET radiations in

デイノコッカス・ラジオデュランスにおける異なるLET放射線による変異頻度の解析

佐藤 勝也; 手島 光平*; 小野寺 威文; 鳴海 一成

Sato, Katsuya; Tejima, Kohei*; Onodera, Takefumi; Narumi, Issei

exhibits extraordinary resistance to the lethal effects of ionizing radiations. This resistance has been attributed to its highly proficient DNA repair capacity. In this study, we investigated antibiotics-resistant mutant frequencies for different LET radiations in . Rifampicin is one of the antibiotics that inhibit RNA synthesis by directly interacting with the RNA polymerase beta subunit protein encoded by gene. By genetically altering the gene in genome, Rif mutant can be generated. The Rif mutant frequencies increased depending on radiation dose. The highest Rif mutant frequencies were observed at a dose range that give 10 to 10 of surviving fraction. There is the possibility that this dose range is the best dose to generate the mutants of interest for research and breeding purposes.